SpeciesReactivity
Mouse

Form
Stds=10-200 ng/ml; Sample=100 ul (diluted); Sensitivity= 5 ng/ml; 105 min assay; Quantitative, Sandwich, 96 tests

Samples
Serum. Plasma, Culture medium and other fluids may be adapted

KitContents
1. Anti-Mouse IgE coated strip plate (8 wells x 12 strips) 6371 2. Mouse IgE Std. A (0 ng/ml), 0.25 ml 6372 3. Mouse IgE Std. B (50 ng/ml), 0.25 ml 6373 4. Mouse IgE Std. C (100 ng/ml), 0. 25 ml 6374 5. Mouse IgE Std. D (200 ng/ml), 0. 25 ml 6375 6. Mouse IgE Std. E (500 ng/ml), 0. 25 ml 6376 7. Mouse IgE Std. F (1000 ng/ml), 0. 25 ml 6377 8. Wash Buffer (100X), 10 ml WB-100 9. Sample Diluent (20x), 10ml SD-20 10. Anti-Mouse IgE-HRP Conjugate (100x), 0.12 ml 6378 11. TMB Substrate, 12 ml 80091 12. Stop solution, 12 ml 80101 13. Instruction Manual M-6370

SpCrossreactivity
The antibodies used in this kit are specific for IgE with no significant reactivity to IgG1-3, or IgA, IgM. Mouse IgE ELISA kit species crossreactivity was tested with various animal sera (IgE). Reactivity above background was observed with goat serum (16%). Rat, Hamster, Guinea Pig, Rabbit, Human, Monkey, Bovine, FBS, Sheep, Pig, and Chicken sera IgE all had less than 1% reactivity

MSDS
TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates

For research use only, not for diagnostic or therapeutic use.